Stereology scholarly articles3/31/2024 ![]() One of the most commonly used methods to estimate nucleic acid concentration is the measurement of sample absorbance at 260 nm. DNA analysis and quantification have become a common process in these laboratories daily as a starting point of the different procedures being performed in the molecular biology laboratory. According to the Eurachem Guide, “The laboratory should use the test and calibration methods, including sampling, that satisfy the clients’ necessities and that are appropriate for the assays being performed.”. Introductionĭiagnostic service laboratories have been experiencing an increasing demand of molecular analysis therefore, the implementation of good laboratory practices and quality assurance has become necessary. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4☌). Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Microvolumes were used to analyse DNA samples. This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA.
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